pEGFP-C1-Ataxin3Q84

Backbone

• Vector backbone

  pEGFP-C1

• Backbone manufacturer

  Clontech

• Backbone size w/o insert (bp)4731

• Vector type

  Mammalian Expression

• Selectable markers

  Neomycin (select with G418) ; G418

Growth in Bacteria

• Bacterial Resistance(s)

  Kanamycin, 50 μg/mL

• Growth Temperature

  37°C

• Growth Strain(s)

  DH5alpha

• Copy number

  High Copy

Gene/Insert

• Gene/Insert name

  Ataxin 3

• Species

  H. sapiens (human)

• Insert Size (bp)

  1300

• Entrez Gene

  ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)

• Tag / Fusion Protein

• EGFP (N terminal on backbone)

Cloning Information

• Cloning methodRestriction Enzyme

• 5′ cloning siteBamHI (unknown if destroyed)

• 3′ cloning siteBamHI (unknown if destroyed)

• 5′ sequencing primerEGFP-C

• 3′ sequencing primerSV40pA-R

• (Common Sequencing Primers)

Resource Information

• Articles Citing this Plasmid

• 22 References

Terms and Licenses

• Academic/Nonprofit Terms

• UBMTA

• Industry Terms

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

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