PX330S-2

pX330S-2(Plasmid #58778)

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• Purpose

  Expresses Cas9 nuclease and gRNA

• Depositing Lab

  Takashi Yamamoto

• Publication

  Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.( How to cite )

• Sequence Information

• Sequences (5)

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Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone

  pUC ori vector

• Vector type

  Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s)

  Spectinomycin, 50 μg/mL

• Growth Temperature

  37°C

• Growth Strain(s)

  Stbl3

• Copy number

  High Copy

Gene/Insert

• Gene/Insert name

  humanized S. pyogenes Cas9 nuclease

• Alt name

  SpCas9

• Alt name

  hSpCas9

• Species

  S. pyogenes

• Insert Size (bp)

  4272

• PromoterCBh

• Tag / Fusion Protein

• 3xFLAG (N terminal on insert)

Cloning Information

• Cloning methodUnknown

• 5′ sequencing primeractatcatatgcttaccgtaac

• (Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by

  Feng Zhang

• Articles Citing this Plasmid

• 25 References

Terms and Licenses

• Academic/Nonprofit Terms

• UBMTA

• Industry Terms

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.